Demonstration of SCAR marker use against the panel of mutants. In each gel lane 1 is a wild-type plant, lanes 2–8 are seven mutants in LOA (134, 125, 165, 152, 146, 135, and 132), lanes 9–15 are seven mutants in LOP (179, 138, 143, 116, 171, 144, and 156), and lane 16 is the dual mutant 168. The markers used are listed to the left of each gel.