Fig. 2.

Ligation of CD46 on naïve T cells alters polarization and signaling in response to TCR stimulation. (Ai) Naïve CTL (untreated or CD46-ligated) were incubated with beads coated with 5 μg/ml mAb to CD3 and CD28 and immunolabeled for CD46 and tubulin. (Scale bar: 10 μm.) (Aii) Quantitation of MTOC polarization to the bead interface. Mean ± SD is shown (50 conjugates scored per sample in triplicate). (B) Naïve CTL (untreated or CD46-ligated) were incubated with beads (antibody coated or not) for 16 h, and the supernatant was assayed for IFN-γ. Mean ± SD of triplicate samples is shown and is representative of three experiments using cells from different donors.