Fig. 4.

Inhibition of histamine-induced vascular permeability by ANGPTL4. VP was evaluated by performing a Miles assay induced by histamine in nude mice electrotransferred with ANGPTL4 or control plasmid. Spectrophotometric analysis of vascular leakiness with Evans blue dye was performed. For each group, extravasation of dye after injection of histamine (1 nM, 10 nM) was compared with extravasation of dye after injection of PBS. Mean nanograms of Evans blue per milligram of fresh tissue are represented ± SEM.