Skip to main content
. 2006 Nov 27;103(49):18739–18744. doi: 10.1073/pnas.0604083103

Fig. 2.

Fig. 2.

Immunoreactivity of NAC-1 in ovarian cancer tissues. (A) Immunoprecipitation/Western blot analyses using NAC-1 and V5 antibodies in RK3E cells transfected with pCDNA6-NAC-1/V5 (RK3E-C1) or vector-only control (Vec). A discrete band corresponding to NAC-1 protein mass is identified in this reciprocal analysis. (B–D) The NAC-1 immunointensity is undetectable or weak in normal ovarian surface epithelium (B) but is strong in a high-grade serous carcinoma (C and D). (E and F) Immunofluorescence of NAC-1 protein localization in ovarian cancer cells in a tissue section. Tumor cells contain NAC-1 protein, which is located in discrete nuclear bodies (E). The adjacent stromal cells are negative for NAC-1 immunoreactivity. A higher magnification demonstrates the NAC-1 nuclear bodies by using a confocal fluorescence microscope (F). (G) Ultrastructure of NAC-1 bodies. ImmunoGold labeling of NAC-1-expressing RK3E cells demonstrates electron-dense bodies decorated by gold particles in the nuclear matrix.