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. 2006 Nov 22;103(49):18775–18780. doi: 10.1073/pnas.0608785103

Fig. 1.

Fig. 1.

Deep hibernation affects dendritic morphology of CA3 pyramidal neurons. (A and B) Effect of deep hibernation (Hib) and induced awakening (Awake 2 and 3 h) on the number of dendritic branch points (A) and total dendritic length (B) of pyramidal neurons from hippocampal CA3 subregion. (C) A representative Golgi stained CA3 pyramidal cell. The image was captured on a Zeiss Axioplan 2i microscope fitted with a Plan Neofluar ×10/0.3 N.A. objective lens and a Hamamatsu Orca ER cooled CCD camera by using MetaVue acquisition software. A stack of 24 images was captured at 2-μm intervals, and the image was displayed as an average projection. (Scale bar: 50 μm.) ∗∗ and ∗∗∗ are P < 0.05 and P < 0.001, respectively, compared with active controls. One-way ANOVA, Tukey post hoc test. Bars represent means plus SEM.