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. 2006 Oct 4;103(49):18828–18833. doi: 10.1073/pnas.0605777103

Fig. 3.

Fig. 3.

Activation and recognition phenotypes of Rx mutants. Leaves of 21-day-old N. tabacum plants were infiltrated with Agrobacterium cultures containing constructs for expression of 35S:Rx mutants (A), Rx mutants with CP-PoMV (B and C), and Rx mutants together with CP-KR (D). Conductivity (μS·cm−1) measurements were derived from samples processed at the indicated time (h) postinfiltration. In C and D, the mutants had one or more of the M1–M4 mutations. Thus, M123 carried the M1, M2, and M3 mutations. In A, the 35S:AT3 construct was a constitutive gain of function mutant (D543E) described (29) that is shown for reference. In C, AB is Agrobacterium infiltration buffer only. For each data point, a minimum of five independent replicates were sampled. Results are shown as the mean ± SEM.