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. 2006 Oct 4;103(49):18828–18833. doi: 10.1073/pnas.0605777103

Fig. 4.

Fig. 4.

Virus-resistance phenotypes of Rx mutants. N. benthamiana plants homozygous for wild-type Rx, M1, M2, or M123 transgenes were inoculated with PVX-HB (A and B) or PoMV (C and D). A and C show symptoms at 20 d postinoculation (dpi), and B and D show Northern analysis of the respective viral RNAs at 10 dpi. Viral RNA accumulation was assessed by RNA gel-blot analysis of 5 μg of total RNA extracted from the appropriate leaves by using probes derived from full-length CP-KR or CP-PoMV PCR products, respectively. The specific lines used were M1 (line 2B-10), M2 [line GR5-F4 in A–D, GR5-16 in D (right-hand lane)], or M123 (lines GR3F-11 in A and C and GR3F11 and GR3-14 in B and D, left to right, respectively).