Fig. 3.

Fine-mapping of QVE7 to an 8.5-kb region of chromosome 3. (A) Summary of the most informative NILs used to fine-map the location of the γ-tocopherol QTL, QVE7. The Cvi allele of QVE7 confers increased γ-tocopherol levels; Ler DNA in recombinants is indicated by shaded regions. The Cvi genomic region common to all lines that have an increased γ-tocopherol phenotype is between 92,660 and 101,100 bp on the top of chromosome 3. The top scale is in kilobases. (B) Predicted gene structures and polymorphisms of At3g01300 and At3g01310. Sequence data were obtained for Ler and Cvi and were aligned to the published Col sequence. The black rectangles indicate predicted exons, and the ribbon tail and arrow indicate the predicted first and final exons of each gene, respectively. Black flags indicate predicted translational start sites; the black octagons indicate stop codons. The white flag indicates the alternate start site for At3g01310 modeled by the Munich Information Center for Protein Sequence. Vertical lines below the diagram indicate the position of polymorphisms between Ler and Cvi; those where Ler differs from both Col and Cvi are marked with a cross. Bold arrows indicate polymorphisms conferring amino acid changes or splice-site-junction alterations that are most likely to confer a phenotypic change. The associated numbers correspond to the polymorphisms numbered in Tables 3 and 5.