Table 1.
Summary of the seed tocopherol QTL found in Arabidopsis
| RIL name | Trait | LG | Position, cM | Supporting interval, cM | lod score | % Explan | High parent | Known biosynthetic genes in interval |
|---|---|---|---|---|---|---|---|---|
| Cvi/Ler | ||||||||
| QVE3 | α-tocopherol | 1 | 88 | 86–90 | 3.15 | 9.0 | Cvi | VTE4, MECPS |
| QVE7 | γ-tocopherol | 3 | 0 | 0–4 | 2.88 | 8.3 | Cvi | IPP2 |
| QVE9 | γ-tocopherol | 5 | 2 | 0–6 | 4.39 | 12.9 | Ler | VTE5 |
| QVE10 | γ-tocopherol | 5 | 14 | 12–16 | 5.81 | 15.9 | Cvi | — |
| QVE12 | γ-tocopherol | 5 | 64 | 62–66 | 3.82 | 10.8 | Cvi | — |
| Col/Ler | ||||||||
| QVE1 | α-tocopherol | 1 | 10 | 8–12 | 9.46 | 40.5 | Col | HPPD |
| QVE2 | α-tocopherol | 1 | 50 | 46–52 | 5.42 | 25.4 | Ler | — |
| QVE4* | δ-tocopherol | 2 | 40 | 34–44 | 7.14 | 32.1 | Col | VTE2, TAT3 |
| QVE5 | γ-tocopherol | 2 | 44 | 40–46 | 5.44 | 25.5 | Col | VTE2, TAT3 |
| QVE6 | α-tocopherol | 2 | 50 | 46–54 | 4.33 | 20.9 | Ler | VTE2, TAT3, CMK |
| QVE8 | γ-tocopherol | 3 | 4 | 0–8 | 3.51 | 17.7 | Col | IPP2 |
| QVE11 | δ-tocopherol | 5 | 36 | 32–42 | 2.94 | 14.7 | Col | — |
| QVE13 | γ-tocopherol | 5 | 76 | 72–80 | 10.87 | 44.5 | Ler | — |
| QVE14* | δ-tocopherol | 5 | 92 | 88–96 | 7.63 | 33.9 | Ler | — |
All values were obtained by using PLABQTL composite interval mapping. LG, linkage group; % Explan, percentage of the phenotypic variance explained by a putative QTL (R2%); MECPS, 2-C-methylerythritol-2,4-cyclodiphosphate synthase; TAT3, tyrosine aminotransferase; CMK, 4-(cytidine-5′-diphospho)-2-C-methylerythritol kinase; —, no candidate.
*The only epistasis observed was between QVE4 and QVE14.