Fig. 3.

Stability kinetics of rhodopsin (Rho) in different surfactants. Thermal stability of rhodopsin was measured as a rate of the decay at A₅₀₀. The A₅₀₀ spectra at different time points are expressed as percentage of absorbance at the initial state of the experiment. (A) The half-life of rhodopsin in different surfactants (as described in the Fig. 2 legend) was as follows: 71 min in 1% OG, 90 min in 0.9% PEG/1% OG, 173 min in 2.5 mM A₆D/1% OG, and 99 min in 2.8 mM RAD8/1% OG. (B) Stability of rhodopsin as a function of the concentration of the peptide surfactant A₆D at 40°C. Half-life of rhodopsin was as follows: 277 min in 3.75 mM A₆D/1% OG, 173 min in 2.5 mM A₆D/1% OG, 101 min in 1.25 mM A₆D/1% OG, and 71 min in 1% OG (PBS). (C) Stability of rhodopsin in DM with or without A₆D at 50°C. Half-life of rhodopsin was as follows: not available in 2.5 mM A₆D/9.8 mM DM, 96 min in 19.6 mM DM, 96 min in 12.3 mM DM, and 102 min in 9.8 mM DM.