Fig. 4.

Determination of the binding affinity of SspB₂ and SspB^154–165 to ZBD₂ using dual polarization interferometry. (A) Open circles represent the experimental binding data of SspB^154–165 to ZBD₂, whereas the solid line represents the theoretical fit to the data assuming a single binding event. (B) Open circles represent the experimental data for the binding of SspB₂ to ZBD₂, whereas the solid line represents the theoretical fit to the data assuming two independent binding events. Curves drawn with short- and long-dashed lines represent the theoretical binding curves assuming a single binding event with K_d values given in C corresponding to the high- and low-affinity interactions, respectively. (C) Binding parameters of SspB^154–165 or SspB₂ to ZBD₂ obtained from the fits to the experimental data. Values in parentheses are standard deviations.