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Ca2+-dependence of 〈P2〉 (open symbols) and force (filled circles) in muscle fibers containing TnC-BR96–103. (A) Activation in the presence of 5 mM MgATP. (B) Rigor with either 1.0 mM (open triangles) or 0.1 mM (inverted open triangles) free Mg2+. (C) Activation with force inhibition by 0.1 mM BTS. Fitted curves from the single (A and C) or double (B) Hill equation (see text for details); the fit to the 〈P2〉 data in A is reproduced as a dotted curve in B and C.
