Fig. 3.

RT-PCR analysis of POTE–actin fusion mRNA in human breast cancer cell lines. Total RNAs were isolated from different cell lines, and first-strand cDNAs were made by reverse transcriptase reaction. PCR on cDNA from breast cancer cell lines was performed with primers specific for POTE–actin fusion transcript (PA01 and PA07 shown in Fig. 1). The breast cancer cell lines MCF-7, HTB-30, HTB-19, and HTB-20 produce a specific 650-bp product. Primer specific for β-actin transcript was used to validate the integrity of the RNA samples.