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. 2006 Nov 10;103(47):17944–17948. doi: 10.1073/pnas.0607497103

Fig. 4.

Fig. 4.

Effect of transgenic overexpression of Ngb on the size of myocardial infarcts produced by LADA occlusion. (a) Representative TTC-stained sections showing viable (red) and ischemic (white) heart tissue. Evans blue dye (blue) delineates tissue outside the territory of the LADA and, therefore, not at risk for infarction from LADA occlusion. (b) Mean infarct volumes ± SEM, expressed as a percentage of left ventricular (LV) volume and of LV volume at risk from LADA occlusion, and volume at risk as a percentage of total LV volume (n = 5); ∗, P < 0.01; ∗∗, P < 0.001 compared with wild type. (c) H&E-stained sections from nonischemic control (Left) and ischemic (Right) regions of Ngb-overexpressing transgenic mouse heart corresponding to TTC-stained (red) and unstained (white) areas, respectively. (d) eNOS immunoreactivity (green) in myocardium of wild-type (Left) and myocardium (Center), and lung (Right) of Ngb-overexpressing transgenic mice, showing predominant localization to vascular endothelium. Nuclei (blue) are counterstained with DAPI. (e) Western blot of eNOS expression (right arrow) in heart of wild-type and Ngb-overexpressing transgenic (Ngb-Tg) mice.