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. 2006 Nov 6;34(21):6147–6157. doi: 10.1093/nar/gkl737

Figure 5.

Figure 5

Tethering of UPF1 leads to reduced accumulation of target mRNAs. (A) Accumulation of GFP-3′boxB target mRNAs in the leaves of N.benthamiana co-infiltrated with GFP-3′boxB + P14 (lanes 1 and 2), GFP-3′boxB + P14 + λN (lanes 3 and 4), GFP-3′boxB + P14 + UPF1 (lanes 5 and 6) or GFP-3′boxB + P14 + λN-UPF1 (lanes 7 and 8) cultures. GFP-3′boxB mRNA levels of λN, UPF1 or λN-UPF1 co-infiltrated samples are shown relative to the GFP-3′boxB transcript level of the corresponding control (−) sample. (B) The effect of UPF1 tethering to the 5′-UTR of GFP-5′boxB target mRNA. (C and D) The effect of UPF1 tethering to the 3′- (P-3′boxB) or to the 5′-UTR (P-5′boxB) of the target mRNAs. PHA-s was co-infiltrated and used as an internal control. Blot was hybridized with PHA-s. Samples and photos were taken at 3 d.p.i.