Table 2.
Helper plasmid | Form g3p | Phage production titer | Infectability of bacteria bearing plasmid | Notes | Potential use |
---|---|---|---|---|---|
M13cp | Full length | Equal M13K07 | 10% DH5αFT | Most similar to phage produced using standard helper phage | Transfer genetic material. Monovalent phage display. |
M13cp-CT | Truncated | 5−20% M13K07 | Equal DH5αFT | Behaves more like g3p deletion. Only phagemid with recombinant g3p are infectious | Multivalent phage display |
M13cp-dg3 | Absent | 0.1−10% M13K07 | Equal DH5αFT | All phage produced have recombinant g3p | Multivalent phage display |