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. 2006 Dec;34(21):6272–6285. doi: 10.1093/nar/gkl801

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© 2006 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression of Bdbd in various cell lines caused arrest at G₂/M phase. (A) DNA binding of CBF after the expression of Bdbd in various cell lines. Whole cell extracts were prepared at 48 h after infection with Ad-Bdbd and used in a DNA-binding assay. (B) Flow cytometric analysis of various cell lines at 48 h after infection with Ad-Bdbd in the presence or absence of Tc. Expression of Bdbd was induced in the absence of Tc in NIH 3T3 and Saos-2 cells, whereas it was induced in the presence of doxycycline in U2 OS cells. (C) The MOIs used were 1:3 for U2 OS cells, 1:6 for NIH 3T3 cells and 1:9 for Saos-2 cells.