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Selection of zeocin-resistant clones from yeast cells expressing wt IN (A), D116A IN (B) or no IN (C). Yeast cells (109) were grown from 0 to 5 h after transformation with the DNA integration substrate containing the two LTRs under non-selective conditions. Cells were plated on solid medium containing zeocin and after five days the zeocin-resistant clones were recovered. The results of three independent experiments are reported.
