Figure 4.

Overview of the Molecular Rearrangements Leading to Functional Activation of the Transferred aadA Gene in the Nucleus.

Nucleotide positions at break points of deletions and/or insertions and point mutations refer to the start of the nptII or aadA coding regions, with negative numbers denoting positions upstream and positive numbers indicating positions downstream of the first nucleotide of the respective coding region (EMBL accession number AM235741). As in Figure 1, prokaryotic-type (chloroplast) sequences are shown in green, whereas eukaryotic-type sequences are in red. Δ indicates a deletion, point mutations are shown by the respective nucleotide substitution (in blue), and the nucleotide sequence of the insertion in Nt-GT31-A2 is shown underneath the map. Promoters and terminators are abbreviated as in Figure 1. Short directly repeated sequences at deletion break points are also indicated. The line Nt-GT31-A1 has the second break point within the translation initiation codon, the two remaining nucleotides of which are underlined. See text for details.