Table 1.
Selection for Activation of the Chloroplast-Type aadA Gene in the Nucleus following Transfer from the Plastid to the Nuclear Genome
| Line | Zygosity | Number of Selection Plates | Selected Spec/Strp- Resistant Lines | Confirmed Lines with aadA Activation |
|---|---|---|---|---|
| Nt-GT16 | Heterozygous | 130 | 2 | 0 |
| Nt-GT21 | Homozygous | 137 | 8 | 4 |
| Nt-GT31 | Heterozygous | 161 | 6 | 4 |
| Total | 428 (= 5564 explants) | 16 | 8 |
Note that lines Nt-GT16 and Nt-GT31 are hetereozygous for the nptII-aadA cassettes in the nucleus (Figure 1A), whereas line Nt-GT21 is homozygous.