Table 2.
Segregation of the Spectinomycin/Streptomycin Resistance in the Progeny from Crosses of Gene Transfer Lines That Were Selected for Functional Activation of the Transferred aadA Gene in the Nucleus
| Line | Cross | Expected Segregation | Observed Segregation | |
|---|---|---|---|---|
| Nt-GT21-A3 | GT × wild type | 1:1 | 92:85 | (1.08:1) |
| GT × GT | 3:1 | 734:299 | (2.45:1) | |
| Wild type × GT | 1:1 | 136:120 | (1.13:1) | |
| Nt-GT21-A5 | GT × wild type | 1:1 | 320:304 | (1.05:1) |
| GT × GT | 3:1 | 317:110 | (2.88:1) | |
| Wild type × GT | 1:1 | 350:389 | (0.90:1) | |
| Nt-GT21-A6 | GT × wild type | 1:1 | 152:139 | (1.09:1) |
| GT × GT | 3:1 | 266:90 | (2.96:1) | |
| Wild type × GT | 1:1 | 314:313 | (1:1) | |
| Nt-GT21-A7 | GT × wild type | 1:1 | 137:117 | (1.17:1) |
| Nt-GT31-A1 | GT × wild type | 1:1 | 295:264 | (1.11:1) |
| GT × GT | 3:1 | 230:74 | (3.11:1) | |
| Wild type × GT | 1:1 | 162:176 | (0.92:1) | |
| Nt-GT31-A2 | GT × wild type | 1:1 | 280:270 | (1.04:1) |
| GT × GT | 3:1 | 607:212 | (2.86:1) | |
| Wild type × GT | 1:1 | 309:279 | (1.11:1) | |
| Nt-GT31-A3 | GT × wild type | 1:1 | 1060:824 | (1.29:1) |
| GT × GT | 3:1 | 780:253 | (3.08:1) | |
| Wild type × GT | 1:1 | 663:653 | (1.02:1) | |
| Nt-GT31-A4 | GT × wild type | 1:1 | 664:665 | (1:1) |
| GT × GT | 3:1 | 287:88 | (3.26:1) | |
| Wild type × GT | 1:1 | 215:205 | (1.05:1) | |
Line Nt-GT21-A7 was male sterile and thus could not be used as pollen donor. GT, gene transfer line.