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. 2006 Nov;18(11):2879–2892. doi: 10.1105/tpc.106.046490

Figure 1.

Figure 1.

Simultaneous Knockout of NRP1 and NRP2 Results in a Short-Root Phenotype.

(A) Genomic structure of NRP1 and NRP2. Boxes indicate the coding sequences. Triangles, T-DNA insertion sites in nrp mutants; arrows, PCR primer location sites.

(B) RT-PCR analysis of NRP1 and NRP2 expression in wild-type, single mutants nrp1-1 and nrp2-1, and double mutant nrp1-1 nrp2-1 plants 14 DAG. ACTIN serves as an internal control.

(C) Wild-type and double mutant nrp1-1 nrp2-1 plants 32 DAG in the in vitro culture medium.

(D) Root growth curves for the wild-type and the double mutant nrp1-1 nrp2-1 plants. The mean value from 20 plants is shown. Error bars represent standard deviations.