Figure 8.

NRP1 Protein Binds Histones H2A and H2B and Chromatin.

(A) Protein–protein interactions examined by pull-down assays. Fractions pulled down by NRP1-coated beads from total protein extracts of transgenic Arabidopsis plants expressing YFP, YFP:NRP1, YFP:NRP2, H2A:YFP, H2B:YFP, or H3:YFP were analyzed by protein gel blotting with a polyclonal anti-GFP antibody (which cross-reacts with YFP). The input fraction represents 5% of the total protein used in pull down. The closed and open arrowheads show the position of YFP and YFP:NRP1/YFP:NRP2, respectively. Positions of the histone fusion proteins H2A:YFP, H2B:YFP, and H3:YFP are indicated by asterisks.

(B) ChIP analysis of NRP1-associated chromatin at different genes. Transgenic Arabidopsis plants expressing H2A:YFP (H) or YFP:NRP1 (N) were analyzed with the anti-GFP antibody. The increasing number of PCR cycles is given at the top of the lanes, and the basal number of cycles (x) is given for each gene at the left of the panels.