Figure 4.

Acetylation State of Histones H3 and H4 of CAB2, RBCS-1A, IAA3, and CHS in Ws, gcn5, and hd1 Seedlings.

Nuclei were extracted from cross-linked 12-d-old light-grown seedlings, sonicated, and immunoprecipitated with antibodies specific to total histone H3, acetylated histone H3, and acetylated histone H4. The immunoprecipitates were analyzed by real-time PCR. The primer sets (arrowheads) are numbered for each gene (open boxes represent the translated regions), and the positions of the primers relative to the initiation ATG codon are indicated. The relative amounts of the PCR products compared with input chromatin from wild-type extracts (arbitrarily given as 100) are shown below the genes. Black bars, acetylated histone H3; white bars, acetylated histone H4. Error bars represent sd values from at least three repetitions.