Figure 6.

GCN5 Was Associated with the Target Light-Responsive Promoters.

(A) Protein gel blot analysis of nuclear extracts from wild-type (Ws) and gcn5 rosette leaves and protein extracts of E. coli transformed by a plasmid expressing GCN5 (I, induced by isopropylthio-β-galactoside; NI, noninduced).

(B) Real-time PCR quantification of RBCS-1A, CAB2, and CHS core promoter regions (as in Figure 4) immunoprecipitated by the GCN5 antibodies or by the preimmune serum. The signals are given as percentage of the input chromatin. Error bars represent sd values from at least three repetitions.