Figure 6.

GAP Is Localized at the Flanks of Pollen Tubes, and Localization Is Dependent on Both the N Terminus and the CRIB Domain.

Pollen tubes were transiently transformed by bombardment with plasmids expressing the indicated YFP fusion proteins and imaged 5 to 7 h later using a confocal laser scanning microscope. Great care was taken to make medial sections through pollen tubes that were growing normally. Constructs expressing partial proteins of RhoGAP1 were as described in the legend to Figure 5. The construct in which the CRIB domain was exchanged with that of Nt RIC1 had amino acids 96 to 126 replaced with the corresponding stretch of Nt RIC1 (YFP-GAP [CRIB-RIC]).