Figure 8.

14-3-3 b-1 Is Expressed in the Cytoplasm of Pollen Tubes.

(A) RNA gel blot probed with 14-3-3 b-1. RNA (5 μg) was loaded per lane and probed with a DIG-labeled probe encompassing the 14-3-3 b-1 coding region. R, roots; S, stems; L, leaves; F, flowers without anthers; P, pollen grains; PT, pollen tubes germinated for 3 h.

(B) Localization of YFP-14-3-3 b-1 in pollen tubes. Pollen tubes were bombarded with plasmids encoding YFP-14-3-3 b-1 and photographed 6 h after bombardment using a confocal microscope. Bar = 10 μm.