Table 1.
Growth Parameters, Photosynthetic Rate, and Pigment Composition of CP24-Depleted Plants
| Photosynthetic Activity (μmol CO2 m−2 s−1)
|
|||||||
|---|---|---|---|---|---|---|---|
| Growth Rate (mm/d) | Flowering Time (d) | Fresh Weight (g) | Chlorophyll (μg/cm2) | Chlorophyll a/b | LL | HL | |
| Wild type | 4.61 ± 0.18 (n = 13) | 35.6 ± 0.83 (n = 14) | 6.86 ± 0.66 (n = 4) | 35.8 ± 1.4 (n = 6) | 3.26 ± 0.04 (n = 6) | 10.3 ± 0.24 (n = 6) | 16.50 ± 0.22 (n = 5) |
| koLhcb6 | 3.85** ±0.16 (n = 13) | 41.4** ±0.65 (n = 9) | 4.82** ±0.46(n = 4) | 33.1 ± 1.3 (n = 6) | 3.31 ± 0.03 (n = 6) | 8.70** ± 0.44 (n = 6) | 15.94 ± 1.22 (n = 5) |
| asLhcb6 | ND | ND | ND | 32.0 ± 1.3 (n = 6) | 3.28 ± 0.11 (n = 6) | 8.25* ± 1.03 (n = 5) | 15.62 ± 1.21 (n = 4) |
| Vio
|
Ant
|
Zea
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Neo | Lut | β-Car | D | L | D | L | D | L | XC | |
| Wild type | 4.68 ± 0.15 | 15.46 ± 0.98 | 10.89 ± 0.34 | 4.13 ± 0.97 | 1.28 ± 0.15 | 0.20 ± 0.05 | 0.43 ± 0.14 | 0.009 ± 0.003 | 2.23 ± 0.36 | 4.33 ± 0.94 |
| koLhcb6 | 4.99 ± 0.25 | 15.31 ± 0.39 | 10.87 ± 0.06 | 4.32 ± 0.22 | 1.10 ± 0.04 | 0.25 ± 0.03 | 0.66 ± 0.05 | 0.007 ± 0.008 | 2.75 ± 0.34 | 4.58 ± 0.20 |
| asLhcb6 | 4.77 ± 0.03 | 15.20 ± 0.83 | 10.60 ± 0.86 | 3.76 ± 1.17 | 1.01 ± 0.39 | 0.24 ± 0.08 | 0.47 ± 0.24 | 0.027 ± 0.024 | 2.23 ± 0.17 | 4.03 ± 1.07 |
Growth rate was the increase in rosette diameter during the linear phase of growth. Flowering time was estimated as when a bolt of 1 cm was produced. Photosynthetic activity was determined at 250 (low light [LL]) and 1500 (high light [HL]) μmol photons m−2 s−1. Values in the top part of the table are ± se, with significant differences indicated by two asterisks (95% confidence limit) or one asterisk (90% confidence limit). Carotenoid content is expressed as mols/100 mols chlorophyll a/b ± sd (n = 3). Neo, neoxanthin; lut, lutein; β-car, β-carotene; vio, violaxanthin; ant, antheraxanthin; zea, zeaxanthin. XC is the total xanthophyll cycle pool. D, dark adapted leaves; L, leaves illuminated with 1500 μmol photons m−2 s−1 for 30 min. There were no significant differences between the D and L values for the nonxanthophyll cycle carotenoids. ND, not determined.