Figure 1.

Gene Expression and Phylogenetic Analyses of NAC Genes.

(A) Preferential expression of a group of NAC genes in stems. The expression data are derived from the AtGenExpress project. Leaves and roots are from 7-day-old plants, and stems are from second internodes of 21-day-old plants.

(B) Phylogenetic analysis of stem-associated NAC genes together with other characterized NAC genes. The branch lengths of the tree are proportional to divergence. The 0.1 scale represents 10% change. Bootstrap values are shown in percentages at nodes.

(C) RT-PCR analysis showing the expression of SND1 in stems but not in other organs. The expression of the EF1α gene was used as an internal control.

(D) Cross section of a stem showing the areas (circled) microdissected by laser for RT-PCR analysis. if, interfascicular fiber; pi, pith cell; xy, xylem bundle.

(E) RT-PCR analysis of laser-microdissected cells showing the expression of SND1 in interfascicular fibers and xylem cells but not in pith cells.