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. Author manuscript; available in PMC: 2007 Oct 1.
Published in final edited form as: Gastroenterology. 2006 Aug 22;131(4):1153–1163. doi: 10.1053/j.gastro.2006.08.022

Figure 5.

Figure 5.

Transgenic TNFR1 expression does not confer TER responses to TNF in the absence of IFN-γ-priming.

A. SDS-PAGE immunoblot of TNFR1 comparing two independently-generated TNFR1-transfected clones and nontransfected control and IFN-γ-primed Caco-2 monolayers. Data are representative of 3 independent experiments.

B. Treatment of TNFR1-transfected monolayers with TNF at doses from 0.1 ng/ml to 10 ng/ml does not induce significant TER loss. Data are mean ± SE of triplicate monolayers normalized to TER of monolayers not treated with TNF. Data are representative of 3 independent experiments.

C. Although TNFR1-transfected monolayers are not responsive to TNF without IFN-γ, they are able to regulate TER in response to TNF, 2.5 ng/ml, after IFN-γ-priming (p<0.02). Data are mean ± SE of triplicate monolayers 8 hours after transfer to TNF. Data were normalized to TER prior to TNF treatments and are representative of 4 independent experiments.