Treatment with TNF at 2.5 ng/ml does not induce apoptosis of TNFR-transfected Caco-2 cells.
A. Increased cleavage of caspase-3 and caspase-9 cleavage are not detected in TNFR1- or TNFR2-transfected 8 hours monolayers after TNF treatment. IFN-γ-primed non-transfected monolayers, without TNF treatment, are shown for reference. Data are representative of 3 independent experiments.
B. Nuclear fragmentation is induced by 100 ng/ml TNF, but not 2.5 ng/ml TNF, in IFN-γ primed monolayers. TNF, 2.5 ng/ml, does not induce nuclear fragmentation in TNFR1-nor TNFR2-transfected monolayers. Data are representative of 3 independent experiments. Bar = 10 μm.