Skip to main content
. Author manuscript; available in PMC: 2007 Oct 1.
Published in final edited form as: Gastroenterology. 2006 Aug 22;131(4):1153–1163. doi: 10.1053/j.gastro.2006.08.022

Figure 8.

Figure 8.

TNF induces occludin redistribution in IFN-γ-primed and TNFR2-expressing Caco-2 cells.

Monolayers of control, IFN-γ-primed, TNFR1-transfected, and TNFR2-transfected Caco-2 cells were treated with TNF, 2.5 ng/ml, as indicated, for 8 hours. Occludin distribution was detected by deconvolution immunofluorescence microscopy. TNF caused irregular occludin undulations at the tight junction and the appearance of intracellular occludin-containing vesicles in IFN-γ-primed and TNFR2-transfected, but not control or TNFR1-transfected monolayers. Bar = 10 μm.