TABLE 1.
Primer sets used for the NS5B site-directed mutagenesis
| Primera | Sequenceb |
|---|---|
| 5B-For | 5′-TCAGCCATATGTCGATGTCGATGTCCTACACATGGACAGGC-3′ |
| 5B-D216Xhis | 5′-TCACTCGAGGCGGGGTCGGGCACGAGACAGGC-3′ |
| L419M F | 5′-ATC ATG TAT GCG CCC ACC ATG TGG GCA AGG ATG ATC CTG -3′ |
| L419M R | 5′-CAG GAT CAT CCT TGC CCA CAT GGT GGG CGC ATA CAT GAT-3′ |
| A421V F | 5′-ATG CGC CCA CCT TGT GGG TAA GGA TGA TCC TGA TG-3′ |
| A421V R | 5′-CAT CAG GAT CAT CCT TAC CCA CAA GGT GGG CGC AT-3′ |
| M423V F | 5′-CTT TGT GGG CAA GGG TGA TTC TGA TGA CTC-3′ |
| M423V R | 5′-GAG TCA TCA GAA TCA CCC TTG CCC ACA AAG-3′ |
| M426T F | 5′-TGG GCA AGG ATG ATC CTG ACC ACT CAT TTC TTC TCC ATC-3′ |
| M426T R | 5′-GAT GGA GAA GAA ATG AGT GGT CAG GAT CAT CCT TGC CCA-3′ |
| M426V F | 5′-CAA GGA TGA TTC TGG TGA CTC ACT TCT TCT C-3′ |
| M426V R | 5′-GAG AAG AAG TGA GTC ACC AGA ATC ATC CTT G-3′ |
a
The nomenclature lists the wild-type residue first and its position in the NS5B protein, followed by the residue it was changed to. F and R, forward and reverse primers, respectively.
b
Mutations are underlined. Restriction sites are indicated in boldface.