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. 2006 Sep 25;50(12):4161–4169. doi: 10.1128/AAC.00433-06

FIG. 4.

FIG. 4.

Kinetics of RNA synthesis by WT and S282T NS5B polymerases, using oligo(C) as a template. (A) RNA products of reactions by either WT or S282T NS5B were resolved in a 14% polyacrylamide-7 M urea gel. RNA markers were synthesized using T7 RNA polymerase as described in Materials and Methods. Each product band is indicated on the right. (B) Comparative quantitation of reaction products from the experiment shown in panel A. Each individual Pi product band from WT or S282T NS5B was quantitated, and the percentage of the ratio of S282T(Pi)/WT(Pi) products was plotted for each i-mer product.