Figure 5.
Agonist-induced currents are inhibited by Gβ5-containing dimers. (A) Sample current traces from G1,4 cell line transfected with 5-HT1A receptor under control conditions (basal) and after agonist treatment (5-HT; 50 μM); 5-HT activates an inwardly rectifying current. (Inset) As compared to control cells, the 5-HT-induced conductance is significantly reduced in cells that coexpress β5-containing dimers (data from cells expressing β5γ2 and β5γ11 are combined). (B, Left) Basal conductance is significantly lower in G1,2m4 cells expressing β5γ2, as compared to control cells. Peak conductance obtained during exposure to 10 μM ACh is reduced in β5γ2-expressing cells. (Right) As compared to control cells, the ACh-induced conductance is significantly reduced in cells that express β5γ2. *, Statistically significant differences from control (P < 0.05 by unpaired t test).