TABLE 1.
MICs of each β-lactam antibiotic for H. pylori 700392 and various transformantsa
| Strain | MIC
|
||||||
|---|---|---|---|---|---|---|---|
| Amoxicillin | Ampicillin | Penicillin G | Ceftriaxone | Cefuroxime | Mezlocillin | Characteristics | |
| 700392 | 0.06 | 0.06 | 0.06 | 0.5 | 0.5 | 1 | ATCC type strain |
| PBP1-T | 0.5 | 0.5 | 0.5 | 0.5 | 1 | 1 | PBP1 transformant |
| HopB-T | 0.25 | 0.5 | 1 | 1 | 1 | 2 | HopB transformant |
| HopC-T | 0.125 | 0.5 | 0.5 | 1 | 0.5 | 1 | HopC transformant |
| PBP1-T/HopB-T | 2 | 4 | 2 | 4 | 2 | 4 | PBP1/HopB double mutant |
| PBP1-T/HopC-T | 1 | 2 | 1 | 2 | 1 | 2 | PBP1/HopC double mutant |
| PBP1/HopB/HopC triple mutant | 4 | 8 | 2 | 2 | 2 | 4 | Triple mutant |
| AmoxR | 4-8 | 4-8 | 4-8 | 4-8 | 4-8 | 2-4 | In vitro-selected strain |
a
MICs were determined after 48 h of incubation at 37°C by a broth microdilution method as described previously (1). The data shown (in μg/ml) are the modes of at least three separate experiments.