Figure 6.

Extension of cell walls from growing petioles of transgenic plants. Native wall specimens from the seventh leaf of 29-day-old T3 plants were clamped at constant load, initially in 50 mM Hepes buffer, pH 6.8, and at 15 min the buffer was exchanged for 50 mM sodium acetate, pH 4.5, to activate expansin-induced extension. These curves are the averages of four, eight, or nine samples for control (C12–5 line), antisense (AS13–1), and sense (S9–1) plants. The antisense curve was significantly different from the control and sense curves (Student's t test, P < 0.05).