TABLE 2.
The average genus and species-level bifidobacterial populations in human feces monitored using FISH-FCM analysis
| Microorganism | Probe | % of microbiota in fecal samples for indicated periodb
|
|||
|---|---|---|---|---|---|
| Prefeeding (0W) | Feeding (2W) | Feeding (4W) | Postfeeding (8W) | ||
| Total bacterial cellsa | Eub338 | 100 | 100 | 100 | 100 |
| Bifidobacterium genus | Bif164m | 12.5 (1.8) | 28.7 (4.5)d | 37.2 (4.2)d | 16.1 (2.6) |
| B. adolescentis | Bado434 | 4.3 (1.1) | 6.9 (1.9) | 9.7 (2.6) | 3.7 (1.2) |
| B. catenulatum group | Bcat187 | 1.8 (0.6) | 3.8 (1.5) | 4.7 (1.7) | 1.7 (0.7) |
| B. longum | Blon1004 | 1.6 (0.7) | 3.3 (0.8) | 5.3 (1.0)d | 1.8 (0.6) |
| B. breve | PBR2 | 0.4 (0.3) | 1.7 (0.4)d | 0.5 (0.2) | 0.04 (0.03) |
| B. bifidum | Bbif186 | 0.2 (0.1) | 0.7 (0.3) | 0.3 (0.2) | 0.1 (0.0) |
| B. dentium | Bden82 | NDc | 0.6 (0.2)d | 0.2 (0.1) | 0.05 (0.03) |
| B. angulatum | Bang198 | ND | 1.0 (0.2)d | 0.7 (0.2)d | 0.5 (0.2) |
| Sum of detected species | 8.3 | 18.0 | 21.4 | 7.9 | |
a
Total bacterial cells are represented as total cells hybridized with probe Eub338.
b
Percentages of bifidobacterial cells in total bacterial cells in fecal samples were enumerated using FISH-FCM analysis. The values are means, with standard errors of means indicated in parentheses (n = 13).
c
ND, not detected.
d
Statistically significant difference (P < 0.05) compared with data for the prefeeding period (0W).