TABLE 2.
Masses of SPUsp45-directed export of NisB-dehydrated therapeutic peptide variants
| Peptide and no. of dehydrations | Sequencea | Dehydrated peptide mass (M + H+) in Dab
|
|
|---|---|---|---|
| Observed | Calculated | ||
| TPPII | ITSISRASVA | ||
| 4 | 3,266 | 3,267 | |
| 3 | 3,284 | 3,285 | |
| 2 | 3,303 | 3,303 | |
| 1 | 3,321 | 3,321 | |
| 0 | 3,339 | 3,339 | |
| LHRH1 | QHWSYGCRPG | ||
| 1 | 3,508 | 3,507 | |
| 0 | 3,527 | 3,525 | |
| LHRH2 | QHWSYSLRCG | ||
| 2 | 3,534 | 3,535 | |
| 1 | 3,552 | 3,553 | |
| 0 | 3,570 | 3,571 | |
| EPO(1-13) | YASHFGPLGWVCK | ||
| 1 | 3,781 | 3,781 | |
| 0 | 3,799 | 3,799 | |
| VP1 | ATFQCAPRG | ||
| 1 | 3,268 | 3,267 | |
| 0 | x | 3,285 | |
| ACTH(1-10) | SYSMECFRWG | ||
| 1 | 3,581 | 3,582 | |
| 0 | 3,598 | 3,600 | |
a
The amino acids that are part of the potentially active part of the peptide are underlined. Amino acids in boldface correspond to mutations.
b
Observed masses are of peptides that contain the nisin leader without Met1. Peptide masses with or without dehydration are shown in Da. x, not observed.