FIG. 4.

Enzymatic degradation of galactan in vitro. Preparations containing either GalA-H (G) or H-LacA (L) were incubated separately or concurrently (G+L) with galactan for 3 h at 37°C, and the products were analyzed by TLC after precipitation and removal of the remaining whole polysaccharide. A negative control treatment using no enzymes (−) was also examined. The amount of product applied to the TLC from the G+L reaction was half that applied from the others (−, L, and G) in order to prevent overloading. Migration patterns of the products were compared to those for stachyose, lactose, and galactose.