TABLE 2.
Primers used for each amplified region of the MS2 genome during real-time RT-PCR
| Localization | Primer(s) | Sequence (5′ to 3′) | Position | Size (in bases) |
|---|---|---|---|---|
| 5′ NCa | 5′NC-f | 5′TGCTCAACTTCCTGTCGAGCT3′ | 24-44 | 111 |
| 5′NC-r | 5′CGCACAGGTCAAACCTCCTAG3′ | 114-134 | ||
| Capsid gene | Cap-f | 5′AAGTGGCAACCCAGACTGTTG3′ | 1534-1554 | 111 |
| Cap-r | 5′GCTCGCAGTCGGAATTCGT3′ | 1626-1644 | ||
| Replicase gene | Rep-f | 5′TTCTCCAACGGTGCTCCTATG3′ | 2145-2165 | 81 |
| Rep-r | 5′GGTAACGGTTGCTTGTTCAGC3′ | 2205-2225 | ||
| 3′ NC | 3′NC-r | 5′TCTTTCGAGCACACCCACC3′ | 3424-3442 | —b |
a
NC, noncoding region.
b
—, only for RT.