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HPLC chromatogram showing UV absorbance at 254 nm of an acidic extract from Mycobacterium sp. strain KMS culture with pyrene-4,5-dione added. The Q1 and Q2 fractions were obtained by using a semi-preparative HPLC column and were identified as phenanthrene-4,5-dicarboxylic acid and 4-phenanthroic acid, respectively.
