TABLE 1.

Identification of proteins induced or newly detected by PAH treatments

Protein no.	Protein identification	NCBI accession no.	Spot density in indicated culture			Mascot score	No. of peptides matched	% Coverage	Observed migrationa		Theoretical migrationb
			Control	Pyrene added	Quinone added				Mr (kDa)	pI	Mr (kDa)	pI
P1	4Fe-4S ferredoxin, iron-sulfur binding	ZP_01282568	0.06	0.25	0.26	153	2	46	16.3	4.3	10.5	4.5
P2	Aromatic-ring-hydroxylating dioxygenase, β subunit	ZP_01282558	NDc	0.15	0.17	569	8	59	16.7	4.4	18.9	4.6
P3	Aromatic-ring-hydroxylating dioxygenase, β subunit	ZP_01286731	ND	0.17	0.13	49	2	14	18.3	4.4	18.8	4.5
P4	Sterol binding protein	ZP_01282557	ND	0.47	0.42	602	20	86	15.6	5.0	14.3	5.0
P5	Hypothetical protein MkmsDRAFT_0077	ZP_01286732	ND	0.66	0.50	637	20	87	16.7	5.3	14.3	5.1
P6	Aromatic-ring-hydroxylating dioxygenase, β subunit	ZP_01286725	ND	0.26	0.22	471	12	59	19.5	4.8	19.5	4.9
P7	Aromatic-ring-hydroxylating dioxygenase, β subunit (nidB2)	AAT51747	ND	1.06	0.68	525	10	60	18.7	5.1	19.4	5.1
P8	Aromatic-ring-hydroxylating dioxygenase, β subunit	ZP_01282769	ND	0.20	0.19	420	7	39	24.9	6.1	22.4	5.7
P9	Phthalate dihydrodiol dehydrogenase	AAQ91917	ND	0.16	0.11	525	10	42	28.6	6.4	28.3	5.5
P10	Ring-hydroxylating dioxygenase, alpha subunit (Rieske [2Fe-2S] region)	ZP_01282559	0.04	0.73	0.50	231	10	17	40.8	6.6	45.0	6.0
P11	Glycosyl hydrolase, BNR	ZP_01282560	0.05	0.45	0.57	812	111	50	40.8	6.4	39.1	5.9
P12	Epoxide hydrolase-like alpha/beta hydrolase fold	ZP_01282545	0.16	0.60	0.50	542	14	46	40.2	6.0	42.4	5.6
P13	Ring-hydroxylating dioxygenase, alpha subunit (Rieske [2Fe-2S] region)	ZP_01282563	0.08	0.59	0.72	1087	25	56	46.4	5.6	50.2	5.3
P14	Aromatic-ring-hydroxylating dioxygenase, alpha subunit (nidA)	AAQ95208	0.03	0.90	0.99	906	16	37	47.0	5.4	50.1	5.3
P15	Flavoprotein-like fumarate reductase/succinate dehydrogenase (FAD- dependent oxidoreductase)	ZP_01282569	0.06	1.70	1.41	1227	23	37	57.8	5.6	73.8	5.8
P16	PhdG (hydratase-aldolase)	AAT51745	ND	0.38	0.50	1160	19	65	36.3	5.2	36.8	5.2
P17	Aldehyde dehydrogenase (NAD+)	ZP_01286724	ND	1.11	1.44	555	8	27	47.0	5.1	52.0	5.0
Q1	Zinc-containing alcohol dehydrogenase superfamily (alcohol dehydrogenase)	ZP_01283066	0.09	0.03	0.36	1468	27	64	41.2	5.1	50.2	5.3
Q2	Cytochrome P450	ZP_01283067	0.21	0.25	3.04	1323	34	65	41.6	5.3	47.6	5.2

^aThe observed migrations M_r and pI were based on 2-D calibration during gel image analysis.

^bThe theoretical migrations M_r and pI were based on Mascot searches.

^cND, nondetectable.