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Hydrolytic activities of wild-type and mutant SSAPs toward Leu-pNA (A), Phe-pNA (B), Arg-pNA (C), and cystinyl-pNA (D). The hydrolytic activities of the wild-type SSAP toward substrates are indicated by black bars and those of mutant enzymes by gray bars. The data are expressed as the means ± standard deviations for three independent experiments. WT, wild type.
