TABLE 3.
Hydrolytic activities of wild-type and high-performance mutant SSAPs toward aminoacyl pNA and OMe derivatives
| Substrate | Activity of WT
|
Mutations of high-performance enzyme | Activity of mutant enzyme
|
||
|---|---|---|---|---|---|
| Va (μmol/min/mg) | Relative to that with pNA | V (μmol/min/mg) | Relative to that with pNA | ||
| Leu-pNA | 68 ± 1.3 | 1 | D198F F221I | 188 ± 2.3 | 1 |
| Leu-OMe | 20 ± 0.9 | 0.3 | D198F F221I | 39 ± 2.6 | 0.2 |
| Arg-pNA | 2.0 ± 0.1 | 1 | D198P F221E | 48 ± 0.5 | 1 |
| Arg-OMe | 2.5 ± 0.2 | 1.3 | D198P F221E | 11 ± 0.5 | 0.2 |
| Cystinyl-pNA | 6.2 ± 0.2 | 1 | D198I F221W | 82 ± 1.8 | 1 |
| Cystinyl-OMe | 0.18 ± 0.04 | 0.03 | D198I F221W | 1.9 ± 0.1 | 0.02 |
a
V, velocity of hydrolytic activity.