FIG. 3.

Southern blot analysis of H. pluvialis transformants P1 to P11 and H. pluvialis WT. The DNA from transformants and WT were digested with XbaI and XhoI and electrophoresed on a 0.8% agarose gel. For detection, a pds-specific cDNA probe (A) or a specific probe for the ampicillin resistance cassette (B) was used. The arrow indicates the position of the 5.78-kb XbaI/XhoI fragment containing the endogenous pds gene. The numbers on the left indicate the sizes of the labeled λ HindIII fragments used as markers (lane M).