FIG. 3.

Modification of the construct containing the whole gene cluster for the transfer into the heterologous host. ░⃞, Start of the biosynthetic cluster with upstream region; ▨, overlapping region; ▪, end of the cluster and downstream region; P, PacI restriction site; Cm, chloramphenicol resistance selection marker; Km, kanamycin resistance selection marker derived from Tn903 and Tn5; Zeo, zeocin resistance selection marker; tet, tetracycline resistance selection marker, ori, p15A origin of replication; oriT, origin of transfer; TA′, fragment of the TA encoding gene from M. xanthus; trpE, homologous target from P. putida; Pm, P. putida toluate catabolic pathway promoter; xylS, regulator of the promoter.