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Insertion into the chromosome. (a) Colony PCR. Lanes: 1 and 2, mutant strain; +, positive control DNA; wt, wild-type M. xanthus DZF1 strain. (b) HPLC-diode array detector chromatogram. Curves: 1, authentic standard reference myxothiazol A; 2, extract of M. xanthus DZF1; 3, extract of M. xanthus DZF1::Pm-mta. Inset panel: UV spectrum of myxothiazol at 314 nm.
