FIG. 5.

SDS-PAGE analysis of purified pyruvate carboxylase preparations. Gels with 7.5% polyacrylamide concentration were used. Lanes M, molecular mass standards. The molecular masses (in kilodaltons) for the standards are shown to the right (A) or left (B) of each gel. (A) Ni-NTA chromatography-purified recombinant P. aeruginosa α₄β₄ PYC from P. aeruginosa BM301(pHL53) (2.3 μg protein). PaPYC, P. aeruginosa PYC. (B) Purified recombinant M. smegmatis α₄-PYC from P. aeruginosa BM301(pHL51). Lanes: Ni, enzyme from Ni-NTA chromatography (10 μg protein); Avi, enzyme from avidin-Sepharose chromatography (3 μg protein).