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. 2006 Oct 13;72(12):7748–7759. doi: 10.1128/AEM.01403-06

FIG. 2.

FIG. 2.

Western blots of the 24 fusion proteins secreted from S. cerevisiae. The same volume of each supernatant was evaluated by Western blotting for each fusion type of a given scFv/scTCR class. The same volume of supernatant was loaded for all scFv/scTCR classes except LWHI (*; 4-fold dilution) and hAb3 (**; 10-fold dilution). Table 1 shows quantitative expression level comparisons. Molecular masses are in kDa, and the solid arrows indicate degradation products. The rightmost panel is a Western blot for the intracellular G3PDH protein. Lanes 1 to 3 are triplicate supernatant samples of type III hAb8-secreting yeast, and lane 4 is a cell extract of type III hAb8-secreting yeast to illustrate the intracellular presence of the G3PDH protein (open arrow).